ATCC Number: CRL-1973 Name: NTERA-2 cl.D1; NT2/D1 Ti


ATCC Number:  CRL-1973
Name:  NTERA-2 cl.D1; NT2/D1
Tissue:  embryonal carcinoma; testis; pluripotent; lung metastasis;
cancer; malignant
Species:  human; male; 22 year old; Caucasian
Depositor:  P.W. Andrews
AnimalStrain:
Receptors:
HeLaMarkers:  no
VirusSuscept:  RA and HMBA TREATED CELLS: human cytomegalovirus
(HCMV); human immunodeficiency virus (HIV-1, HTLV-III)
VirusResist:  UNTREATED CELLS: human cytomegalovirus (HCMV); human
immunodeficiency virus (HIV-1, HTLV-III)
Tumorigenic:
Oncogene:
RevTranscript:
Karyotype:
Morphology:  fibroblast
Isoenzymes:
Isotype:
AntigenExp:
Products:
Growth:  monolayer
Contaminants:
References:  Lab. Invest. 50:147-162, 1984; Dev. Biol. 103:285-293,
1984; Science 224:159-161, 1984; Dev. Biol. 122:21-34, 1987; Biochim.
Biophys. Acta 948:17-36, 1988; Differentiation 37:73-79, 1988;
Differentiation 43:131-138, 1990; J. Virol.  65:2732-2735, 1991
PassageSub:
Medium:  Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%
Price Code:  J
FreezeMedium:
FluidRenewal:  every 2 to 3 days
Subculturing:  Subcultures are prepared by scraping. Cells from
confluent cultures (approximately 20 million cells per 75 sq. cm.) are
dislodged from the flask surface, aspirated and dispensed into new
flasks. Trypsin - EDTA dissociation can be  used, but it is better to
do so infrequently. Cultures should be maintained at high density.
Seed new flasks at a density of at least 5 X 10 exp6 viable cells per
75 sq. cm. flask
SplitRatio:
DoublingTime:
Restrictions:  none
Packing Class:  1
YearAccess:
MinPDL:
MaxPDL:
BioSafety:
Comments:  the NTERA-2 cl.D1 cell line is a pluripotent human
testicular embryonal carcinoma cell line derived by cloning the
NTERA-2 cell line; the parental NTERA-2 lines was established in 1980
from a nude mouse xenograft of the Tera-2 cell  line (see ATCC
HTB-106); this clone differentiates along neuroectodermal lineages
after exposure to retinoic acid (RA) or hexamethylene bisacetamide
((HMBA); the RA induced differentiation is characterized by glycolipid
changes,  appearance of neurons, and induction of homeobox (HOX) gene
clusters; the cells exhibit high expression of N-myc oncogene
activity; to induce differentiation, the cells should be trypsinized
and seeded at a density 1 X 10 exp6 cells  per 75 sq. cm. in medium
containing 0.01 mM trans-retinoic acid; stock solutions of
trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen
(preferably under a nitrogen atmosphere)