ATCC Number: HTB-39 Name: SK-CO-1 Tissue: adenocarc


ATCC Number:  HTB-39
Name:  SK-CO-1
Tissue:  adenocarcinoma; carcinoma; colon; ascites; cancer; colorectal
Species:  human; 65 year old; male; Caucasian
Depositor:  G. Trempe; L.J. Old
AnimalStrain:
Receptors:
HeLaMarkers:  no
VirusSuscept:
VirusResist:
Tumorigenic:
Oncogene:  myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src
-
RevTranscript:
Karyotype:  (P7) hypertriploid to hypotetraploid with abnormalities
including dicentrics, minutes, rings, secondary constrictions, and 8
large submetacentric markers
Morphology:  epithelial
Isoenzymes:  Me-2, 1; PGM3, 1-2; PGM1, 1; ES-D, 1; AK-1, 1-2; GLO-1,
1-2; G6PD, B; Phenotype Frequency Product: 0.0037
Isotype:
AntigenExp:  Blood Type O; Rh+; HLA A1, A3, B7, B13
Products:
Growth:  monolayer
Contaminants:
References:  Fogh, J and Trempe, G; In: Human Tumor Cells In Vitro,
pp. 115-159, J. Fogh (ed.), Plenum Press, New York, 1975; J. Natl.
Cancer Inst. 58:209-214, 1977; J. Natl. Cancer Inst. 66:1003-1012,
1981; Int. J. Cancer 41:287-296, 1988
PassageSub:
Medium:  Minimum essential medium (Eagle) in Earle's BSS with
nonessential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine
serum, 10%
Price Code:  J
FreezeMedium:  Culture medium, 95%; DMSO, 5%
FluidRenewal:  2 to 3 times weekly
Subculturing:  Remove medium, rinse with fresh 0.25% trypsin solution,
remove trypsin and let the culture sit at room temperature (or at 37C)
until the cells detach (about 10 minutes). Add fresh medium, aspirate
and dispense into new flasks.  Subculture every 6 to 8 days
SplitRatio:  A ratio of 1:2 to 1:3 is recommended
DoublingTime:
Required forms:
Restrictions:    The cells are distributed for research purposes only.
The Memorial Sloan-Kettering Cancer Center releases the line subject
to the following: 1.) The cells or their products must not be
distributed to third parties. Commercial interests are the exclusive
property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed
commercial use of these cells must first be negotiated with The
Director, Office of Industrial Affairs, Memorial Sloan-Kettering
Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212)
639-3620; FAX (212) 753-5764.
Packing Class:  1
YearAccess:
MinPDL:
MaxPDL:
BioSafety:
Comments:  ultrastructural features include microvilli,
microfilaments, small mitochondria with dark granules and dilated
cristae, sparse RER, few Golgi, lipid droplets, multilamellar bodies,
no virus particles were detected; the line is  positive for expression
of c-myc, N-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes