ATCC Number: CCL-81 Name: Vero Tissue: kidney; norm


ATCC Number:  CCL-81
Name:  Vero
Tissue:  kidney; normal
Species:  monkey; African green; Cercopithecus aethiops; adult
Depositor:  W.D. Hann; J.S. Rhim
AnimalStrain:
Receptors:
HeLaMarkers:
VirusSuscept:  poliovirus 1, 2, 3; Getah; Ndumu; Pixuna; Ross River;
Semliki Forest; Paramaribo; Kokobera; Modoc; Murutucu; Germiston;
Guaroa; Pongola; Tacaribe; SV-5; SV40; rubeola; rubellavirus; reovirus
1, 2, 3; simian adenoviruses
VirusResist:  Stratford; Apeu; Caraparu; Madrid; Nepuyo; Ossa
Tumorigenic:
Oncogene:
RevTranscript:  reverse transcriptase negative
Karyotype:
Morphology:  epithelial
Isoenzymes:
Isotype:
AntigenExp:
Products:
Growth:  monolayer
Contaminants:
References:  Nippon Rinsho 21:1209, 1963
PassageSub:  113
ATCCMedium:  ATCC Cat No. 30-2003
Medium:  Minimum essential medium Eagle with 2 mM L-glutamine and
Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM
non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal
bovine serum, 10%
Price Code:  J
FreezeMedium:
FluidRenewal:  2 to 3 times weekly
Subculturing:  Remove medium, add fresh 0.25% trypsin solution for 1
to 2 minutes, remove trypsin and allow the culture to stand at room
temperature for 5 to 10 minutes. Add fresh medium, aspirate and
dispense into new flasks
SplitRatio:  A ratio of 1:2 to 1:6 is recommended
DoublingTime:
Restrictions:  none
Packing Class:  1
YearAccess:
MinPDL:
MaxPDL:
BioSafety:
Comments: